Seal, Rebecca P., Ph.D.|
Associate Professor, Neurobiology
Pittsburgh Center for Pain Research
Address: 6058 BST3
3501 Fifth Avenue
Pittsburgh, PA 15213-3301
The Seal laboratory studies the neural circuitry that drives behavior in health and disease. We are currently focused on the following circuits: i) peripheral and central pain circuits, ii) motor circuits affected by Parkinson’s disease iii) glutamate and GABA co-release by forebrain cholinergic neurons and iv) peripheral and central auditory circuits. We aim to discover fundamental principals and mechanisms of the nervous system as well as identify novel treatment strategies for nervous system disorders.
Experimental Approach: We use mice as a model system and techniques such as virally-mediated designer receptors (DREADDs) to selectively activate or inhibit neurons, virally-mediated anterograde and retrograde tools to trace circuits, slice electrophysiology with optogenetic stimulation, confocal and 2-photon microscopy, molecular biology, histology, biochemistry and behavior.
For more details visit the Seal Lab website at seallab.neurobio.pitt.edu.
Select Publications: (For a complete list go to PubMed or Google Scholar)
Peripheral and Central Pain Circuits:
Uncovering the Cells and Circuits of Touch in Normal and Pathological Settings.
A Critical Role for Dopamine D5 Receptors in Pain Chronicity in Male Mice.
Neural circuits for pain: Recent advances and current views.
Illuminating the Gap: Neuronal Cross-Talk within Sensory Ganglia and Persistent Pain.
Do the distinct synaptic properties of VGLUTs shape pain?
Targeting Toll-like receptors to treat chronic pain.
Dorsal Horn Circuits for Persistent Mechanical Pain.
Injury-induced mechanical hypersensitivity requires C-low threshold mechanoreceptors.
Motor Circuits Affected by Parkinson's Disease:
Loss of VGLUT3 Produces Circadian-Dependent Hyperdopaminergia and Ameliorates Motor Dysfunction and l-Dopa-Mediated Dyskinesias in a Model of Parkinson's Disease.
Glutamate and GABA Co-release by Forebrain Chollinergic Neurons:
Layer- and cell type-selective co-transmission by a basal forebrain cholinergic projection to the olfactory bulb.
Striatal Cholinergic Neurotransmission Requires VGLUT3.
Striatal cholinergic interneurons drive GABA release from dopamine terminals.
Restoration of hearing in the VGLUT3 knockout mouse using virally mediated gene therapy.
Sensorineural deafness and seizures in mice lacking vesicular glutamate transporter 3.
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of Pittsburgh Department of Neurobiology